Proteintech anti h2b antibody
$a, Venn diagram showed the overlap of ZCCHC4 PAR-CLIP-seq target genes between two biological replicates. b, Distribution plots (up) and sequence motif enrichment analysis (down) of ZCCHC4-bound histone mRNAs. ZCCHC4-binding motifs were identified by HOMER findMotifsGenome.pl from the PAR-CLIP-seq peaks of two biological replicates. The motif length was restricted to 4-8 nucleotides. The P -value was calculated from the random background sequences with a ZOOPS score. c, RIP/qPCR analyses showed the binding of ZCCHC4 and potential targets in wild-type HeLa cells. d, Volcano plot depicting down-regulated (blue) and up-regulated (red) genes identified by Ribo-seq in ZCCHC4 KO cells compared with WT cells. e, Integrated Genome Viewer (IGV) showing the mapped reads of the binding peaks identified by ribosome-bound fragments identified by Ribo-seq of H2BC6 after ZCCHC4 knockout. The histograms represent the sum of mapped reads along the genome. The mapped reads of Ribo-seq were normalized by the sequencing depth. f, Volcano plot depicting down-regulated (blue) and up-regulated (red) genes identified by TMT in ZCCHC4 KO cells compared with WT cells. g, Cumulative distribution of translation efficiency with histone mRNA (red) and non-histone (blue) mRNA by TMT (up) and Ribo-seq (down). h, Volcano plot depicting replication-dependent (blue, RD-Histone) and replication-independent (red, RI-Histone) histone identified by Ribo-seq (left) and TMT (right) in ZCCHC4 KO cells compared with WT cells. i, Western blot analysis to validate the expression of RD-histone (H2A and <t>H2B)</t> and RI-histone (H2AZ) in ZCCHC4 knockout and control cells. β-actin was used as internal control. j, The broken line chart showed the relative distribution of the mRNA level of <t>H2B,</t> H2AC and H4A in the different fractions isolated by the sucrose gradient. The same volume (50 µL) of the fractions was loaded to perform RT-qPCR. k, RT-qPCR analysis of polysome-or monosome (80S)-bound histone mRNA in WT (left) ZCCHC4 knockout (right) HeLa cells. Note: c , k Two-tailed unpaired Student’s t tests. Data were presented as mean ± SD (n=3). * P <0.05, * * P <0.01, ** * P <0.001, **** P <0.0001. ns: not significant.$
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histone h2b (Santa Cruz Biotechnology)

Santa Cruz Biotechnology histone h2b
$a, Venn diagram showed the overlap of ZCCHC4 PAR-CLIP-seq target genes between two biological replicates. b, Distribution plots (up) and sequence motif enrichment analysis (down) of ZCCHC4-bound histone mRNAs. ZCCHC4-binding motifs were identified by HOMER findMotifsGenome.pl from the PAR-CLIP-seq peaks of two biological replicates. The motif length was restricted to 4-8 nucleotides. The P -value was calculated from the random background sequences with a ZOOPS score. c, RIP/qPCR analyses showed the binding of ZCCHC4 and potential targets in wild-type HeLa cells. d, Volcano plot depicting down-regulated (blue) and up-regulated (red) genes identified by Ribo-seq in ZCCHC4 KO cells compared with WT cells. e, Integrated Genome Viewer (IGV) showing the mapped reads of the binding peaks identified by ribosome-bound fragments identified by Ribo-seq of H2BC6 after ZCCHC4 knockout. The histograms represent the sum of mapped reads along the genome. The mapped reads of Ribo-seq were normalized by the sequencing depth. f, Volcano plot depicting down-regulated (blue) and up-regulated (red) genes identified by TMT in ZCCHC4 KO cells compared with WT cells. g, Cumulative distribution of translation efficiency with histone mRNA (red) and non-histone (blue) mRNA by TMT (up) and Ribo-seq (down). h, Volcano plot depicting replication-dependent (blue, RD-Histone) and replication-independent (red, RI-Histone) histone identified by Ribo-seq (left) and TMT (right) in ZCCHC4 KO cells compared with WT cells. i, Western blot analysis to validate the expression of RD-histone (H2A and <t>H2B)</t> and RI-histone (H2AZ) in ZCCHC4 knockout and control cells. β-actin was used as internal control. j, The broken line chart showed the relative distribution of the mRNA level of <t>H2B,</t> H2AC and H4A in the different fractions isolated by the sucrose gradient. The same volume (50 µL) of the fractions was loaded to perform RT-qPCR. k, RT-qPCR analysis of polysome-or monosome (80S)-bound histone mRNA in WT (left) ZCCHC4 knockout (right) HeLa cells. Note: c , k Two-tailed unpaired Student’s t tests. Data were presented as mean ± SD (n=3). * P <0.05, * * P <0.01, ** * P <0.001, **** P <0.0001. ns: not significant.$
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histone h2b (Novus Biologicals)

Novus Biologicals histone h2b
$a, Venn diagram showed the overlap of ZCCHC4 PAR-CLIP-seq target genes between two biological replicates. b, Distribution plots (up) and sequence motif enrichment analysis (down) of ZCCHC4-bound histone mRNAs. ZCCHC4-binding motifs were identified by HOMER findMotifsGenome.pl from the PAR-CLIP-seq peaks of two biological replicates. The motif length was restricted to 4-8 nucleotides. The P -value was calculated from the random background sequences with a ZOOPS score. c, RIP/qPCR analyses showed the binding of ZCCHC4 and potential targets in wild-type HeLa cells. d, Volcano plot depicting down-regulated (blue) and up-regulated (red) genes identified by Ribo-seq in ZCCHC4 KO cells compared with WT cells. e, Integrated Genome Viewer (IGV) showing the mapped reads of the binding peaks identified by ribosome-bound fragments identified by Ribo-seq of H2BC6 after ZCCHC4 knockout. The histograms represent the sum of mapped reads along the genome. The mapped reads of Ribo-seq were normalized by the sequencing depth. f, Volcano plot depicting down-regulated (blue) and up-regulated (red) genes identified by TMT in ZCCHC4 KO cells compared with WT cells. g, Cumulative distribution of translation efficiency with histone mRNA (red) and non-histone (blue) mRNA by TMT (up) and Ribo-seq (down). h, Volcano plot depicting replication-dependent (blue, RD-Histone) and replication-independent (red, RI-Histone) histone identified by Ribo-seq (left) and TMT (right) in ZCCHC4 KO cells compared with WT cells. i, Western blot analysis to validate the expression of RD-histone (H2A and <t>H2B)</t> and RI-histone (H2AZ) in ZCCHC4 knockout and control cells. β-actin was used as internal control. j, The broken line chart showed the relative distribution of the mRNA level of <t>H2B,</t> H2AC and H4A in the different fractions isolated by the sucrose gradient. The same volume (50 µL) of the fractions was loaded to perform RT-qPCR. k, RT-qPCR analysis of polysome-or monosome (80S)-bound histone mRNA in WT (left) ZCCHC4 knockout (right) HeLa cells. Note: c , k Two-tailed unpaired Student’s t tests. Data were presented as mean ± SD (n=3). * P <0.05, * * P <0.01, ** * P <0.001, **** P <0.0001. ns: not significant.$
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anti acetyl histone h2b (Cell Signaling Technology Inc)

Cell Signaling Technology Inc anti acetyl histone h2b
$a, Venn diagram showed the overlap of ZCCHC4 PAR-CLIP-seq target genes between two biological replicates. b, Distribution plots (up) and sequence motif enrichment analysis (down) of ZCCHC4-bound histone mRNAs. ZCCHC4-binding motifs were identified by HOMER findMotifsGenome.pl from the PAR-CLIP-seq peaks of two biological replicates. The motif length was restricted to 4-8 nucleotides. The P -value was calculated from the random background sequences with a ZOOPS score. c, RIP/qPCR analyses showed the binding of ZCCHC4 and potential targets in wild-type HeLa cells. d, Volcano plot depicting down-regulated (blue) and up-regulated (red) genes identified by Ribo-seq in ZCCHC4 KO cells compared with WT cells. e, Integrated Genome Viewer (IGV) showing the mapped reads of the binding peaks identified by ribosome-bound fragments identified by Ribo-seq of H2BC6 after ZCCHC4 knockout. The histograms represent the sum of mapped reads along the genome. The mapped reads of Ribo-seq were normalized by the sequencing depth. f, Volcano plot depicting down-regulated (blue) and up-regulated (red) genes identified by TMT in ZCCHC4 KO cells compared with WT cells. g, Cumulative distribution of translation efficiency with histone mRNA (red) and non-histone (blue) mRNA by TMT (up) and Ribo-seq (down). h, Volcano plot depicting replication-dependent (blue, RD-Histone) and replication-independent (red, RI-Histone) histone identified by Ribo-seq (left) and TMT (right) in ZCCHC4 KO cells compared with WT cells. i, Western blot analysis to validate the expression of RD-histone (H2A and <t>H2B)</t> and RI-histone (H2AZ) in ZCCHC4 knockout and control cells. β-actin was used as internal control. j, The broken line chart showed the relative distribution of the mRNA level of <t>H2B,</t> H2AC and H4A in the different fractions isolated by the sucrose gradient. The same volume (50 µL) of the fractions was loaded to perform RT-qPCR. k, RT-qPCR analysis of polysome-or monosome (80S)-bound histone mRNA in WT (left) ZCCHC4 knockout (right) HeLa cells. Note: c , k Two-tailed unpaired Student’s t tests. Data were presented as mean ± SD (n=3). * P <0.05, * * P <0.01, ** * P <0.001, **** P <0.0001. ns: not significant.$
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cell signaling technology 8135 (Cell Signaling Technology Inc)

Cell Signaling Technology Inc cell signaling technology 8135
$a, Venn diagram showed the overlap of ZCCHC4 PAR-CLIP-seq target genes between two biological replicates. b, Distribution plots (up) and sequence motif enrichment analysis (down) of ZCCHC4-bound histone mRNAs. ZCCHC4-binding motifs were identified by HOMER findMotifsGenome.pl from the PAR-CLIP-seq peaks of two biological replicates. The motif length was restricted to 4-8 nucleotides. The P -value was calculated from the random background sequences with a ZOOPS score. c, RIP/qPCR analyses showed the binding of ZCCHC4 and potential targets in wild-type HeLa cells. d, Volcano plot depicting down-regulated (blue) and up-regulated (red) genes identified by Ribo-seq in ZCCHC4 KO cells compared with WT cells. e, Integrated Genome Viewer (IGV) showing the mapped reads of the binding peaks identified by ribosome-bound fragments identified by Ribo-seq of H2BC6 after ZCCHC4 knockout. The histograms represent the sum of mapped reads along the genome. The mapped reads of Ribo-seq were normalized by the sequencing depth. f, Volcano plot depicting down-regulated (blue) and up-regulated (red) genes identified by TMT in ZCCHC4 KO cells compared with WT cells. g, Cumulative distribution of translation efficiency with histone mRNA (red) and non-histone (blue) mRNA by TMT (up) and Ribo-seq (down). h, Volcano plot depicting replication-dependent (blue, RD-Histone) and replication-independent (red, RI-Histone) histone identified by Ribo-seq (left) and TMT (right) in ZCCHC4 KO cells compared with WT cells. i, Western blot analysis to validate the expression of RD-histone (H2A and <t>H2B)</t> and RI-histone (H2AZ) in ZCCHC4 knockout and control cells. β-actin was used as internal control. j, The broken line chart showed the relative distribution of the mRNA level of <t>H2B,</t> H2AC and H4A in the different fractions isolated by the sucrose gradient. The same volume (50 µL) of the fractions was loaded to perform RT-qPCR. k, RT-qPCR analysis of polysome-or monosome (80S)-bound histone mRNA in WT (left) ZCCHC4 knockout (right) HeLa cells. Note: c , k Two-tailed unpaired Student’s t tests. Data were presented as mean ± SD (n=3). * P <0.05, * * P <0.01, ** * P <0.001, **** P <0.0001. ns: not significant.$
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histone 2b antibody (Novus Biologicals)

Novus Biologicals histone 2b antibody
$a, Venn diagram showed the overlap of ZCCHC4 PAR-CLIP-seq target genes between two biological replicates. b, Distribution plots (up) and sequence motif enrichment analysis (down) of ZCCHC4-bound histone mRNAs. ZCCHC4-binding motifs were identified by HOMER findMotifsGenome.pl from the PAR-CLIP-seq peaks of two biological replicates. The motif length was restricted to 4-8 nucleotides. The P -value was calculated from the random background sequences with a ZOOPS score. c, RIP/qPCR analyses showed the binding of ZCCHC4 and potential targets in wild-type HeLa cells. d, Volcano plot depicting down-regulated (blue) and up-regulated (red) genes identified by Ribo-seq in ZCCHC4 KO cells compared with WT cells. e, Integrated Genome Viewer (IGV) showing the mapped reads of the binding peaks identified by ribosome-bound fragments identified by Ribo-seq of H2BC6 after ZCCHC4 knockout. The histograms represent the sum of mapped reads along the genome. The mapped reads of Ribo-seq were normalized by the sequencing depth. f, Volcano plot depicting down-regulated (blue) and up-regulated (red) genes identified by TMT in ZCCHC4 KO cells compared with WT cells. g, Cumulative distribution of translation efficiency with histone mRNA (red) and non-histone (blue) mRNA by TMT (up) and Ribo-seq (down). h, Volcano plot depicting replication-dependent (blue, RD-Histone) and replication-independent (red, RI-Histone) histone identified by Ribo-seq (left) and TMT (right) in ZCCHC4 KO cells compared with WT cells. i, Western blot analysis to validate the expression of RD-histone (H2A and <t>H2B)</t> and RI-histone (H2AZ) in ZCCHC4 knockout and control cells. β-actin was used as internal control. j, The broken line chart showed the relative distribution of the mRNA level of <t>H2B,</t> H2AC and H4A in the different fractions isolated by the sucrose gradient. The same volume (50 µL) of the fractions was loaded to perform RT-qPCR. k, RT-qPCR analysis of polysome-or monosome (80S)-bound histone mRNA in WT (left) ZCCHC4 knockout (right) HeLa cells. Note: c , k Two-tailed unpaired Student’s t tests. Data were presented as mean ± SD (n=3). * P <0.05, * * P <0.01, ** * P <0.001, **** P <0.0001. ns: not significant.$
Histone 2b Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lg histoneh2b type 2e novus bio nbp3 (Novus Biologicals)

Novus Biologicals lg histoneh2b type 2e novus bio nbp3
$a, Venn diagram showed the overlap of ZCCHC4 PAR-CLIP-seq target genes between two biological replicates. b, Distribution plots (up) and sequence motif enrichment analysis (down) of ZCCHC4-bound histone mRNAs. ZCCHC4-binding motifs were identified by HOMER findMotifsGenome.pl from the PAR-CLIP-seq peaks of two biological replicates. The motif length was restricted to 4-8 nucleotides. The P -value was calculated from the random background sequences with a ZOOPS score. c, RIP/qPCR analyses showed the binding of ZCCHC4 and potential targets in wild-type HeLa cells. d, Volcano plot depicting down-regulated (blue) and up-regulated (red) genes identified by Ribo-seq in ZCCHC4 KO cells compared with WT cells. e, Integrated Genome Viewer (IGV) showing the mapped reads of the binding peaks identified by ribosome-bound fragments identified by Ribo-seq of H2BC6 after ZCCHC4 knockout. The histograms represent the sum of mapped reads along the genome. The mapped reads of Ribo-seq were normalized by the sequencing depth. f, Volcano plot depicting down-regulated (blue) and up-regulated (red) genes identified by TMT in ZCCHC4 KO cells compared with WT cells. g, Cumulative distribution of translation efficiency with histone mRNA (red) and non-histone (blue) mRNA by TMT (up) and Ribo-seq (down). h, Volcano plot depicting replication-dependent (blue, RD-Histone) and replication-independent (red, RI-Histone) histone identified by Ribo-seq (left) and TMT (right) in ZCCHC4 KO cells compared with WT cells. i, Western blot analysis to validate the expression of RD-histone (H2A and <t>H2B)</t> and RI-histone (H2AZ) in ZCCHC4 knockout and control cells. β-actin was used as internal control. j, The broken line chart showed the relative distribution of the mRNA level of <t>H2B,</t> H2AC and H4A in the different fractions isolated by the sucrose gradient. The same volume (50 µL) of the fractions was loaded to perform RT-qPCR. k, RT-qPCR analysis of polysome-or monosome (80S)-bound histone mRNA in WT (left) ZCCHC4 knockout (right) HeLa cells. Note: c , k Two-tailed unpaired Student’s t tests. Data were presented as mean ± SD (n=3). * P <0.05, * * P <0.01, ** * P <0.001, **** P <0.0001. ns: not significant.$
Lg Histoneh2b Type 2e Novus Bio Nbp3, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti acetylated histone h2b (Cell Signaling Technology Inc)

Cell Signaling Technology Inc anti acetylated histone h2b
$a, Venn diagram showed the overlap of ZCCHC4 PAR-CLIP-seq target genes between two biological replicates. b, Distribution plots (up) and sequence motif enrichment analysis (down) of ZCCHC4-bound histone mRNAs. ZCCHC4-binding motifs were identified by HOMER findMotifsGenome.pl from the PAR-CLIP-seq peaks of two biological replicates. The motif length was restricted to 4-8 nucleotides. The P -value was calculated from the random background sequences with a ZOOPS score. c, RIP/qPCR analyses showed the binding of ZCCHC4 and potential targets in wild-type HeLa cells. d, Volcano plot depicting down-regulated (blue) and up-regulated (red) genes identified by Ribo-seq in ZCCHC4 KO cells compared with WT cells. e, Integrated Genome Viewer (IGV) showing the mapped reads of the binding peaks identified by ribosome-bound fragments identified by Ribo-seq of H2BC6 after ZCCHC4 knockout. The histograms represent the sum of mapped reads along the genome. The mapped reads of Ribo-seq were normalized by the sequencing depth. f, Volcano plot depicting down-regulated (blue) and up-regulated (red) genes identified by TMT in ZCCHC4 KO cells compared with WT cells. g, Cumulative distribution of translation efficiency with histone mRNA (red) and non-histone (blue) mRNA by TMT (up) and Ribo-seq (down). h, Volcano plot depicting replication-dependent (blue, RD-Histone) and replication-independent (red, RI-Histone) histone identified by Ribo-seq (left) and TMT (right) in ZCCHC4 KO cells compared with WT cells. i, Western blot analysis to validate the expression of RD-histone (H2A and <t>H2B)</t> and RI-histone (H2AZ) in ZCCHC4 knockout and control cells. β-actin was used as internal control. j, The broken line chart showed the relative distribution of the mRNA level of <t>H2B,</t> H2AC and H4A in the different fractions isolated by the sucrose gradient. The same volume (50 µL) of the fractions was loaded to perform RT-qPCR. k, RT-qPCR analysis of polysome-or monosome (80S)-bound histone mRNA in WT (left) ZCCHC4 knockout (right) HeLa cells. Note: c , k Two-tailed unpaired Student’s t tests. Data were presented as mean ± SD (n=3). * P <0.05, * * P <0.01, ** * P <0.001, **** P <0.0001. ns: not significant.$
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rabbit histone h2b (Novus Biologicals)

Novus Biologicals rabbit histone h2b
$a, Venn diagram showed the overlap of ZCCHC4 PAR-CLIP-seq target genes between two biological replicates. b, Distribution plots (up) and sequence motif enrichment analysis (down) of ZCCHC4-bound histone mRNAs. ZCCHC4-binding motifs were identified by HOMER findMotifsGenome.pl from the PAR-CLIP-seq peaks of two biological replicates. The motif length was restricted to 4-8 nucleotides. The P -value was calculated from the random background sequences with a ZOOPS score. c, RIP/qPCR analyses showed the binding of ZCCHC4 and potential targets in wild-type HeLa cells. d, Volcano plot depicting down-regulated (blue) and up-regulated (red) genes identified by Ribo-seq in ZCCHC4 KO cells compared with WT cells. e, Integrated Genome Viewer (IGV) showing the mapped reads of the binding peaks identified by ribosome-bound fragments identified by Ribo-seq of H2BC6 after ZCCHC4 knockout. The histograms represent the sum of mapped reads along the genome. The mapped reads of Ribo-seq were normalized by the sequencing depth. f, Volcano plot depicting down-regulated (blue) and up-regulated (red) genes identified by TMT in ZCCHC4 KO cells compared with WT cells. g, Cumulative distribution of translation efficiency with histone mRNA (red) and non-histone (blue) mRNA by TMT (up) and Ribo-seq (down). h, Volcano plot depicting replication-dependent (blue, RD-Histone) and replication-independent (red, RI-Histone) histone identified by Ribo-seq (left) and TMT (right) in ZCCHC4 KO cells compared with WT cells. i, Western blot analysis to validate the expression of RD-histone (H2A and <t>H2B)</t> and RI-histone (H2AZ) in ZCCHC4 knockout and control cells. β-actin was used as internal control. j, The broken line chart showed the relative distribution of the mRNA level of <t>H2B,</t> H2AC and H4A in the different fractions isolated by the sucrose gradient. The same volume (50 µL) of the fractions was loaded to perform RT-qPCR. k, RT-qPCR analysis of polysome-or monosome (80S)-bound histone mRNA in WT (left) ZCCHC4 knockout (right) HeLa cells. Note: c , k Two-tailed unpaired Student’s t tests. Data were presented as mean ± SD (n=3). * P <0.05, * * P <0.01, ** * P <0.001, **** P <0.0001. ns: not significant.$
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z na (Novus Biologicals)

Novus Biologicals z na
$a, Venn diagram showed the overlap of ZCCHC4 PAR-CLIP-seq target genes between two biological replicates. b, Distribution plots (up) and sequence motif enrichment analysis (down) of ZCCHC4-bound histone mRNAs. ZCCHC4-binding motifs were identified by HOMER findMotifsGenome.pl from the PAR-CLIP-seq peaks of two biological replicates. The motif length was restricted to 4-8 nucleotides. The P -value was calculated from the random background sequences with a ZOOPS score. c, RIP/qPCR analyses showed the binding of ZCCHC4 and potential targets in wild-type HeLa cells. d, Volcano plot depicting down-regulated (blue) and up-regulated (red) genes identified by Ribo-seq in ZCCHC4 KO cells compared with WT cells. e, Integrated Genome Viewer (IGV) showing the mapped reads of the binding peaks identified by ribosome-bound fragments identified by Ribo-seq of H2BC6 after ZCCHC4 knockout. The histograms represent the sum of mapped reads along the genome. The mapped reads of Ribo-seq were normalized by the sequencing depth. f, Volcano plot depicting down-regulated (blue) and up-regulated (red) genes identified by TMT in ZCCHC4 KO cells compared with WT cells. g, Cumulative distribution of translation efficiency with histone mRNA (red) and non-histone (blue) mRNA by TMT (up) and Ribo-seq (down). h, Volcano plot depicting replication-dependent (blue, RD-Histone) and replication-independent (red, RI-Histone) histone identified by Ribo-seq (left) and TMT (right) in ZCCHC4 KO cells compared with WT cells. i, Western blot analysis to validate the expression of RD-histone (H2A and <t>H2B)</t> and RI-histone (H2AZ) in ZCCHC4 knockout and control cells. β-actin was used as internal control. j, The broken line chart showed the relative distribution of the mRNA level of <t>H2B,</t> H2AC and H4A in the different fractions isolated by the sucrose gradient. The same volume (50 µL) of the fractions was loaded to perform RT-qPCR. k, RT-qPCR analysis of polysome-or monosome (80S)-bound histone mRNA in WT (left) ZCCHC4 knockout (right) HeLa cells. Note: c , k Two-tailed unpaired Student’s t tests. Data were presented as mean ± SD (n=3). * P <0.05, * * P <0.01, ** * P <0.001, **** P <0.0001. ns: not significant.$
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beclin1 s30 (Cell Signaling Technology Inc)

Cell Signaling Technology Inc beclin1 s30
$a, Venn diagram showed the overlap of ZCCHC4 PAR-CLIP-seq target genes between two biological replicates. b, Distribution plots (up) and sequence motif enrichment analysis (down) of ZCCHC4-bound histone mRNAs. ZCCHC4-binding motifs were identified by HOMER findMotifsGenome.pl from the PAR-CLIP-seq peaks of two biological replicates. The motif length was restricted to 4-8 nucleotides. The P -value was calculated from the random background sequences with a ZOOPS score. c, RIP/qPCR analyses showed the binding of ZCCHC4 and potential targets in wild-type HeLa cells. d, Volcano plot depicting down-regulated (blue) and up-regulated (red) genes identified by Ribo-seq in ZCCHC4 KO cells compared with WT cells. e, Integrated Genome Viewer (IGV) showing the mapped reads of the binding peaks identified by ribosome-bound fragments identified by Ribo-seq of H2BC6 after ZCCHC4 knockout. The histograms represent the sum of mapped reads along the genome. The mapped reads of Ribo-seq were normalized by the sequencing depth. f, Volcano plot depicting down-regulated (blue) and up-regulated (red) genes identified by TMT in ZCCHC4 KO cells compared with WT cells. g, Cumulative distribution of translation efficiency with histone mRNA (red) and non-histone (blue) mRNA by TMT (up) and Ribo-seq (down). h, Volcano plot depicting replication-dependent (blue, RD-Histone) and replication-independent (red, RI-Histone) histone identified by Ribo-seq (left) and TMT (right) in ZCCHC4 KO cells compared with WT cells. i, Western blot analysis to validate the expression of RD-histone (H2A and <t>H2B)</t> and RI-histone (H2AZ) in ZCCHC4 knockout and control cells. β-actin was used as internal control. j, The broken line chart showed the relative distribution of the mRNA level of <t>H2B,</t> H2AC and H4A in the different fractions isolated by the sucrose gradient. The same volume (50 µL) of the fractions was loaded to perform RT-qPCR. k, RT-qPCR analysis of polysome-or monosome (80S)-bound histone mRNA in WT (left) ZCCHC4 knockout (right) HeLa cells. Note: c , k Two-tailed unpaired Student’s t tests. Data were presented as mean ± SD (n=3). * P <0.05, * * P <0.01, ** * P <0.001, **** P <0.0001. ns: not significant.$
Beclin1 S30, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/histone+h2b+antibody/Acetyl-Histone+H2B+(Lys12)+Antibody/pm41786876-75-8-51
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anti histone h2b (Novus Biologicals)

Novus Biologicals anti histone h2b
$a, Venn diagram showed the overlap of ZCCHC4 PAR-CLIP-seq target genes between two biological replicates. b, Distribution plots (up) and sequence motif enrichment analysis (down) of ZCCHC4-bound histone mRNAs. ZCCHC4-binding motifs were identified by HOMER findMotifsGenome.pl from the PAR-CLIP-seq peaks of two biological replicates. The motif length was restricted to 4-8 nucleotides. The P -value was calculated from the random background sequences with a ZOOPS score. c, RIP/qPCR analyses showed the binding of ZCCHC4 and potential targets in wild-type HeLa cells. d, Volcano plot depicting down-regulated (blue) and up-regulated (red) genes identified by Ribo-seq in ZCCHC4 KO cells compared with WT cells. e, Integrated Genome Viewer (IGV) showing the mapped reads of the binding peaks identified by ribosome-bound fragments identified by Ribo-seq of H2BC6 after ZCCHC4 knockout. The histograms represent the sum of mapped reads along the genome. The mapped reads of Ribo-seq were normalized by the sequencing depth. f, Volcano plot depicting down-regulated (blue) and up-regulated (red) genes identified by TMT in ZCCHC4 KO cells compared with WT cells. g, Cumulative distribution of translation efficiency with histone mRNA (red) and non-histone (blue) mRNA by TMT (up) and Ribo-seq (down). h, Volcano plot depicting replication-dependent (blue, RD-Histone) and replication-independent (red, RI-Histone) histone identified by Ribo-seq (left) and TMT (right) in ZCCHC4 KO cells compared with WT cells. i, Western blot analysis to validate the expression of RD-histone (H2A and <t>H2B)</t> and RI-histone (H2AZ) in ZCCHC4 knockout and control cells. β-actin was used as internal control. j, The broken line chart showed the relative distribution of the mRNA level of <t>H2B,</t> H2AC and H4A in the different fractions isolated by the sucrose gradient. The same volume (50 µL) of the fractions was loaded to perform RT-qPCR. k, RT-qPCR analysis of polysome-or monosome (80S)-bound histone mRNA in WT (left) ZCCHC4 knockout (right) HeLa cells. Note: c , k Two-tailed unpaired Student’s t tests. Data were presented as mean ± SD (n=3). * P <0.05, * * P <0.01, ** * P <0.001, **** P <0.0001. ns: not significant.$
Anti Histone H2b, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/histone+h2b+antibody/Histone+H2B+%5Bac+Lys11%5D+Antibody+(RM456)/pmc02034663-445-51-53
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anti histone h2b - by Bioz Stars, 2026-10

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$a, Venn diagram showed the overlap of ZCCHC4 PAR-CLIP-seq target genes between two biological replicates. b, Distribution plots (up) and sequence motif enrichment analysis (down) of ZCCHC4-bound histone mRNAs. ZCCHC4-binding motifs were identified by HOMER findMotifsGenome.pl from the PAR-CLIP-seq peaks of two biological replicates. The motif length was restricted to 4-8 nucleotides. The P -value was calculated from the random background sequences with a ZOOPS score. c, RIP/qPCR analyses showed the binding of ZCCHC4 and potential targets in wild-type HeLa cells. d, Volcano plot depicting down-regulated (blue) and up-regulated (red) genes identified by Ribo-seq in ZCCHC4 KO cells compared with WT cells. e, Integrated Genome Viewer (IGV) showing the mapped reads of the binding peaks identified by ribosome-bound fragments identified by Ribo-seq of H2BC6 after ZCCHC4 knockout. The histograms represent the sum of mapped reads along the genome. The mapped reads of Ribo-seq were normalized by the sequencing depth. f, Volcano plot depicting down-regulated (blue) and up-regulated (red) genes identified by TMT in ZCCHC4 KO cells compared with WT cells. g, Cumulative distribution of translation efficiency with histone mRNA (red) and non-histone (blue) mRNA by TMT (up) and Ribo-seq (down). h, Volcano plot depicting replication-dependent (blue, RD-Histone) and replication-independent (red, RI-Histone) histone identified by Ribo-seq (left) and TMT (right) in ZCCHC4 KO cells compared with WT cells. i, Western blot analysis to validate the expression of RD-histone (H2A and H2B) and RI-histone (H2AZ) in ZCCHC4 knockout and control cells. β-actin was used as internal control. j, The broken line chart showed the relative distribution of the mRNA level of H2B, H2AC and H4A in the different fractions isolated by the sucrose gradient. The same volume (50 µL) of the fractions was loaded to perform RT-qPCR. k, RT-qPCR analysis of polysome-or monosome (80S)-bound histone mRNA in WT (left) ZCCHC4 knockout (right) HeLa cells. Note: c , k Two-tailed unpaired Student’s t tests. Data were presented as mean ± SD (n=3). * P <0.05, * * P <0.01, ** * P <0.001, **** P <0.0001. ns: not significant.$

Journal: bioRxiv

Article Title: ZCCHC4 Promotes Translation of Replication-dependent Histone mRNAs by Recruiting Cytoplasmic eIF3 complex

doi: 10.1101/2025.06.21.660898

Figure Lengend Snippet: a, Venn diagram showed the overlap of ZCCHC4 PAR-CLIP-seq target genes between two biological replicates. b, Distribution plots (up) and sequence motif enrichment analysis (down) of ZCCHC4-bound histone mRNAs. ZCCHC4-binding motifs were identified by HOMER findMotifsGenome.pl from the PAR-CLIP-seq peaks of two biological replicates. The motif length was restricted to 4-8 nucleotides. The P -value was calculated from the random background sequences with a ZOOPS score. c, RIP/qPCR analyses showed the binding of ZCCHC4 and potential targets in wild-type HeLa cells. d, Volcano plot depicting down-regulated (blue) and up-regulated (red) genes identified by Ribo-seq in ZCCHC4 KO cells compared with WT cells. e, Integrated Genome Viewer (IGV) showing the mapped reads of the binding peaks identified by ribosome-bound fragments identified by Ribo-seq of H2BC6 after ZCCHC4 knockout. The histograms represent the sum of mapped reads along the genome. The mapped reads of Ribo-seq were normalized by the sequencing depth. f, Volcano plot depicting down-regulated (blue) and up-regulated (red) genes identified by TMT in ZCCHC4 KO cells compared with WT cells. g, Cumulative distribution of translation efficiency with histone mRNA (red) and non-histone (blue) mRNA by TMT (up) and Ribo-seq (down). h, Volcano plot depicting replication-dependent (blue, RD-Histone) and replication-independent (red, RI-Histone) histone identified by Ribo-seq (left) and TMT (right) in ZCCHC4 KO cells compared with WT cells. i, Western blot analysis to validate the expression of RD-histone (H2A and H2B) and RI-histone (H2AZ) in ZCCHC4 knockout and control cells. β-actin was used as internal control. j, The broken line chart showed the relative distribution of the mRNA level of H2B, H2AC and H4A in the different fractions isolated by the sucrose gradient. The same volume (50 µL) of the fractions was loaded to perform RT-qPCR. k, RT-qPCR analysis of polysome-or monosome (80S)-bound histone mRNA in WT (left) ZCCHC4 knockout (right) HeLa cells. Note: c , k Two-tailed unpaired Student’s t tests. Data were presented as mean ± SD (n=3). * P <0.05, * * P <0.01, ** * P <0.001, **** P <0.0001. ns: not significant.

Article Snippet: The corresponding antibodies included anti-ZCCHC4 antibody with 1:1000 dilution (Abcam, ab209901), anti-RPS6 antibody with 1:1000 dilution (Proteintech, 66886-1-Ig), anti-RPL14 antibody with 1:1000 dilution (Proteintech, 14991-1-AP), anti-CDKN1A antibody with 1:1000 dilution (Proteintech, 10355-1-AP), anti-puromycin antibody with 1:1000 dilution (ABclonal, A21205), anti-H2B antibody with 1:2000 dilution (PTM-1007), anti-H2A antibody with 1:2000 dilution (ABclonal, A3692), anti-H2AZ antibody with 1:2000 dilution (Proteintech, 16441-1-AP), anti-HistoneH1.2 antibody with 1:1000 dilution (Proteintech, 19649-1-AP), anti HistoneH3 antibody with 1:2000 dilution (ABclonal, A22348), anti-α-Tubulin antibody with 1:3000 dilution (Proteintech, 80762-1-RR), anti-EIF3H antibody with 1:1000 dilution (Proteintech, 11310-1-AP), anti-EIF3A antibody with 1:1000 dilution (Proteintech, 26178-1-AP), anti-PDCD4 antibody with 1:1000 dilution (Proteintech, 12587-1-AP), anti-CyclinB1 antibody with 1:1000 dilution (ABclonal, A19037), anti-GAPDH antibody with 1:5000 dilution (Proteintech, 10494-1-AP), anti-β-actin antibody with 1:3000 dilution (SAB Signalway Antibody, 52901), and anti-Flag antibody with 1:2000 dilution (Sigma, F1804).

Techniques: Sequencing, Binding Assay, Knock-Out, Western Blot, Expressing, Control, Isolation, Quantitative RT-PCR, Two Tailed Test

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